Clinical significance of serum neutrophil gelatinase-associated lipocalin detection in early diagnosis of acute kidney injury

Abstract

Abstract: Objective To investigate the clinical significance of serum neutrophil gelatinase-associated lipocalin(NGAL) detection in early diagnosis of acute kidney injury(AKI). Methods A total of 60 inpatients in intensive care unit (ICU) and coronary care unit (CCU) without chronic kidney diseases were enrolled. Sera were collected before surgery and 6, 12 and 48 h after surgery respectively. There were 27 AKI patients and other 33 patients without AKI. The concentration of NGAL was determined by enzyme-linked immunosorbent assay (ELISA). Serum creatinine (SCr) was detected by Jaffé reaction method. Serum cystatin C (Cys C) was detected by Latex-enhanced immunoturbidimetry. AKI was defined as an increase in SCr of at least 50% from baseline within 48 h. The efficiency of NGAL in early diagnosis of AKI was evaluated by receiver operating characteristic (ROC) curve. Results Serum NGAL increased significantly 6, 12 and 48 h after surgery， while serum Cys C increased 12 and 48 h after surgery, and SCr increased 48 h after surgery in the AKI group. There was no obvious difference in the concentrations of NGAL, SCr and Cys C in the non-AKI group before and 6, 12 and 48 h after surgery (P>0.05). By ROC curve analysis, serum NGAL 6 h after surgery had an area under the curve of 0.51 ［95% confidence interval (CI): 0.25-0.78］， serum NGAL 12h after surgery had an area under the curve of 0.80 (95%CI: 0.58-0.95)， and serum NGAL 48 h after surgery had an area under the curve of 0.85 (95%CI: 0.61-0.99). 〖Conclusions Serum NGAL increases 6 h after surgery and predicts AKI accurately 12 h after surgery. For predicting AKI, serum NGAL is earlier than Cys C and SCr， and shows that serum NGAL can be as potential marker for the early diagnosis of AKI.

CAI Leiming;LI Qian;JIN Caifeng;TAN Longyi;LIAO Minlei;BAO Jie. Clinical significance of serum neutrophil gelatinase-associated lipocalin detection in early diagnosis of acute kidney injury[J]. , 2012, 27(4): 287-290.

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