Diagnostic significance of lipoarabinomannan antigen in pleural fluid for tuberculous pleurisy

Abstract

Abstract: Objective　To investigate whether the determination of lipoarabinomannan (LAM) antigen in pleural fluid may be used as an indicator for the diagnosis of tuberculous pleurisy. Methods　Double antibody sandwich enzymelinked immunosorbent assay(ELISA) was performed to detect LAM antigen in 96 pleural fluid samples (46 patients with tuberculous pleurisy and 50 patients with nontuberculous pleurisy). Results　The positive rate of LAM antigen in pleural fluid with tuberculous pleurisy group was 54%， and the positive rate in pleural fluid with nontuberculous pleurisy was 22%. There was a statistical difference between them(P<0.05). Conclusions　LAM antigen could be used as a new indicator for the diagnosis of tuberculous pleurisy.

Key words: Lipoarabinomannan, Pleural fluid, Tuberculous pleurisy

WANG Fang;YUE Jun;PENG Rong. Diagnostic significance of lipoarabinomannan antigen in pleural fluid for tuberculous pleurisy[J]. , 2012, 27(3): 199-201.

References

［1］Gopi A， Madhavan SM， Sharma SK， et al. Diagnosis and treatment of tuberculous pleural effusion in 2006［J］. Chest， 2007， 131(3): 880-889.

［2］Trajman A， Pai M， Dheda K， et al. Novel tests for diagnosing tuberculous pleural effusion: what works and what does not［J］. Eur Respir J， 2008， 31(5): 1098-1106.

［3］Sharma SK， Banga A. Pleural fluid interferon-gamma and adenosine deaminase levels in tuberculosis pleural effusion: a cost-effectiveness analysis［J］. J Clin Lab Anal， 2005， 19(2): 40-46.

［4］Greco S， Girardi E， Masciangelo R， et al. Adenosine deaminase and interferon gamma measurements for the diagnosis of tuberculous pleurisy: a meta-analysis［J］. Int J Tuberc Lung Dis， 2003， 7(8): 777-786.

［5］孙忠顺，管波清，古淑香,等.血清抗脂阿拉伯甘露糖及38 kD抗体检测对涂阴肺结核及肺外结核诊断价值［J］.中国防痨杂志，2002，24(3):140-142.

［6］刘宗道，肖雪飞，孙圣华，等.血清LAM-IgG与PPD-IgG检测对肺结核的诊断价值［J］.医学临床研究，2002，19(3):231-232.

［7］马　玙，高薇薇，赵丰增.快速酶联免疫吸附法检测血清脂阿拉伯甘露糖-IgG对肺结核的诊断价值［J］.新医学，1996，28(7):360.

［8］Shah M, Variava E, Holmes CB, et al. Diagnostic accuracy of a urine lipoarabinomannan test for tuberculosis in hospitalized patients in a high HIV prevalence setting［J］. J Acquir Immune Defic Syndr， 2009， 52(2):145-151.

［9］Pereira Arias- Bouda LM， Nguyen LN， Ho LM， et al. Development of antigen detection assay for diagnosis of tuberculosis using sputum samples［J］. J Clin Microbiol， 2000， 38(6):2278-2283.

［10］Patel VB, Bhigiee AI, Paruk HF, et al. Utility of a novel lipoarabinomannan assay for the diagnosis of tuberculous meningitis in a resource-poor high-HIV prevalence setting［J］. Cerebrospinal Fluid Res， 2009，6:13.

［11］Sada E， Aguilar D， Torres M. Detection of lipoara-binomannan as a diagnostic test for tuberculosis［J］. J Clin Microbiol， 1992， 30(9):2415-2418.

［12］陈　蕾，闻　平.胸腔积液ADA、CRP和LAM-IgG联合检测的鉴别诊断价值［J］.现代检验医学杂志，2005，20(4):23-25.

[1]	SHEN Jie, JIA Li, DONG Xiaoshuai, MENG Cheng. Different methods for the rapid diagnosis of tuberculous pleurisy [J]. Laboratory Medicine, 2019, 34(3): 221-224.
[2]	WANG Binjie, HU Li, CUI Tianpen, WANG Yueqin. Significance on different sample sources of cytopathological combined determination in the diagnosis of lung cancer [J]. Laboratory Medicine, 2016, 31(1): 13-16.
[3]	LI Duofu, CHEN Yulin, XIA Yu. Evaluation on the clinical diagnosis efficiency of pleural fluid ADA and LDH for tuberculous pleuritis [J]. Laboratory Medicine, 2015, 30(10): 987-990.
[4]	DU Yanqing 1，FENG Shuang 2，FENG Ranran 2，LIU Shuye 1. The significance of T-SPOT.TB testin the diagnosis of tuberculous pleurisy [J]. , 2012, 27(12): 1070-1073.