Abstract:   Objective　To evaluate the consistency of glycosylated serum protein（GSP） testing results between 2 biochemical analytical systems，Dada and Olympus. Methods　Each biochemical analytical system was separately calibrated and ensured the daily internal quality control under control limits. According to Clinical and Laboratory Standards Institute（CLSI） GP29-A document，the split-sample testing（SST） was carried on. Each serum sample，whose concentration was selected in the methodology measurable range，was divided into 2 parts and made duplicate testingfor GSP，which could notprovide proficiency testing（PT） on external quality assessment，on the each biochemical analytical system. The mean（〖AKx-D〗），difference（d） and allowable difference values（D） werefinally calculated to evaluate the results′ consistency. Results　The GSP results on Olympus detection system were within the allowable differences of Dade detection system（〖AKx-D〗±D）.Two biochemical analytical systems had good correlation of GSP testing in the concentration rangefrom 1.0 mmol/L to 16.0 mmol/L，and their results′ consistency was accepted. Conclusions　All the results could be accepted between the 2 biochemical analytical systems，and one of them could be selected to testGSP. SSTis a convenientandfastalternative assessmentprocedure to evaluate the consistencyfor GSP testing results，whose proficiency testing is notavailable atpresent.

Key words: Glycosylated serum protein, Split-sample testing, Analytical system, Resultconsistency