Abstract:

Objective To investigate the relationship between the expressions of serum miR-9-5p and miR-185-5p in patients with ischemic heart failure（IHF）and cardiac function and ventricular remodeling. Methods From February 2021 to February 2023，108 patients with IHF who received treatment at Nanyang Central Hospital were enrolled. According to New York Heart Association（NYHA）cardiac function classification，the patients with IHF were classified into grade Ⅰ-Ⅱ group（35 cases），grade Ⅲ group（42 cases） and grade Ⅳ group（31 cases）. In addition，50 patients with mild coronary heart disease in remission period without heart failure were randomly enrolled as control group. The levels of serum miR-9-5p，miR-185-5p，B-type natriuretic peptide（BNP），left ventricular ejection fraction（LVEF），left atrial diameter（LAD）and left ventricular end diastolic diameter（LVEDD）were determined and measured. Pearson correlation analysis was used to evaluate the correlation of serum miR-9-5p，miR-185-5p and cardiac function，ventricular remodeling. Receiver operating characteristic（ROC）curve was used to analyze the diagnostic values of serum miR-9-5p，miR-185-5p for the diagnosis of IHF. Results The miR-9-5p and miR-185-5p relative expressions，LAD，LVEDD and BNP in IHF group were higher than those in control group（P<0.05），and LVEF was lower than that in control group（P<0.05）. The levels of LAD，LVEDD，BNP，miR-9-5p and miR-185-5p relative expressions were increased in grade Ⅰ-Ⅱ，Ⅲ，Ⅳ groups（P<0.001），and LVEF was decreased（P<0.001）. LAD，LVEDD，BNP and miR-9-5p，miR-185-5p had positive correlation（P<0.05）. LVEF had negative correlation（P<0.05）. The miR-9-5p and miR-185-5p were risk factors for IHF[odds ratios（OR）were 2.114 and 2.224，95% confidence intervals（CI）were 1.129-3.958 and 1.297-3.813，P<0.05]. The areas under curves （AUC） of miR-9-5p，miR-185-5p，LVEF，BNP were 0.806，0.789，0.734 and 0.751，respectively. The AUC of miR-9-5p+miR-185-5p+LVEF，miR-9-5p+miR-185-5p+BNP，miR-9-5p+miR-185-5p+LVEF+BNP were 0.879，0.876，0.936，respectively. Conclusions The expression levels of miR-9-5p and miR-185-5p in serum of patients with IHF are increased，they are related to cardiac dysfunction and may be involved in ventricular remodeling. The combination of serum miR-9-5p，miR-185-5p，LVEF and BNP has high efficacy in the diagnosis of IHF.

Key words: MiR-9-5p, MiR-185-5p, Ischemic heart failure, Cardiac function, Ventricular remodeling