Effect of long non-coding RNA SNHG5 on the transcriptome of human hypopharyngeal carcinoma cells and clinical significance

doi:10.3969/j.issn.1673-8640.2022.03.018

Laboratory Medicine ›› 2022, Vol. 37 ›› Issue (3): 281-287.DOI: 10.3969/j.issn.1673-8640.2022.03.018

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Effect of long non-coding RNA SNHG5 on the transcriptome of human hypopharyngeal carcinoma cells and clinical significance

ZHOU Huan¹, TANG Ying², DU Cuiping², YANG Yang², FENG Huijie², QIU Miaoxin², YAN Peiyi³, CAI Xiaoyao⁴, JIN Shu¹()

1. Hengyang Medical School,University of South China,Hengyang 421001,Hunan,China
2. Department of Otolaryngology,Shanghai Putuo District People's Hospital,Shanghai 200060,China
3. Department of Clinical Laboratory,Shanghai Putuo District People's Hospital,Shanghai 200060,China
4. Department of Central Laboratory,Shanghai Putuo District People's Hospital,Shanghai 200060,China

Received:2021-10-17 Revised:2022-02-17 Online:2022-03-30 Published:2022-05-10
Contact: JIN Shu

Abstract

Abstract:

Objective To investigate the effect of long non-coding RNA（lncRNA） small nucleolar RNA host gene 5（SNHG5） on the transcriptome of human hypopharyngeal carcinoma cell line FaDu. Methods The rLV-hSNHG5-ZsGreen-Puro lentivirus and rLV-ZsGreen-Puro control lentivirus were constructed. FaDu cells infected with rLV-hSNHG5-ZsGreen-Puro lentivirus were served as rLV-SNHG5 group,and those infected with rLV-ZsGreen-Puro control lentivirus were served as rLV group. Differential gene sets were screened,and the gene transcriptional ability,Gene Ontology（GO） function,Kyoto Encyclopedia of Genes and Genomes（KEGG） signaling pathway,Reactome pathway and Disease Ontology（DO） function were analyzed. Results The relative expression of SNHG5 in rLV-SNHG5 group was higher than that in rLV group（P<0.01）. A total of 1 070 differentially expressed genes were screened by gene differential analysis,of which 537 were up-regulated,and 533 were down-regulated. The top 10 up-regulated genes with the most significant difference were LCP1,NT5E,CTGF,AXL,AKAP12,PXDN,THBS1,4-Mar,TMEM200A and RIPOR3,and the top 10 down-regulated genes were PKP1,SERPINB13,KLK10,AC011473.4,NOTCH3,CA2,EGLN3,SLC6A8,KRT4 and VAV3. Functional analysis showed that SNHG5 changed the gene transcriptional ability of some genes in FaDu cells and participated in the regulation of GO function,KEGG signaling pathway,Reactome pathway and DO function. Conclusions SNHG5 has a certain effect on the transcriptome of FaDu cells.

Key words: Small nucleolar RNA host gene 5, Long non-coding RNA, Transcriptomics, Hypopharyngeal carcinoma

CLC Number:

R393

ZHOU Huan, TANG Ying, DU Cuiping, YANG Yang, FENG Huijie, QIU Miaoxin, YAN Peiyi, CAI Xiaoyao, JIN Shu. Effect of long non-coding RNA SNHG5 on the transcriptome of human hypopharyngeal carcinoma cells and clinical significance[J]. Laboratory Medicine, 2022, 37(3): 281-287.

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References 17

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Effect of long non-coding RNA SNHG5 on the transcriptome of human hypopharyngeal carcinoma cells and clinical significance

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