Abstract:

Objective To investigate the application of serum hyaluronan（HA） and hyaluronan synthetase 2（HAS2） in bone tumor. Methods A total of 35 patients with bone tumor,40 patients with bone infection and 60 healthy subjects were enrolled. The expression levels of HA,HAS2 and HAS2 mRNA were determined. Receiver operating characteristic（ROC）curve was used to analyze the diagnostic efficiency of each marker. Results The expression levels of HA,HAS2 and HAS2 mRNA in patients with bone tumor before and after surgery were significantly higher than those in healthy subjects（P<0.05）. The expression levels of HAS2 and HAS2 mRNA in patients with bone tumor before and after surgery were significantly higher than those in patients with bone infection（P<0.05）,but there was no significant difference in serum HA level between them（P>0.05）. After bone tumors were removed,the expression levels of HA,HAS2 and HAS2 mRNA were significantly reduced（P<0.05）. ROC curve analysis showed that the area under the curve（AUC） of HA,Has2 and Has2 mRNA single detection and combined detection were 0.888,0.651,0.747 and 0.956,respectively,when healthy subjects were taken as control;and the AUC were 0.485,0.782,0.630 and 0.798,respectively,when bone infection group was taken as control. Conclusions The combined determination of HA ,HAS2 and HAS2 mRNA plays a role in the auxiliary diagnosis and therapeutic monitoring of bone tumor.

Key words: Hyaluronan, Hyaluronan synthetase 2, Bone tumor