Homemade Sephadex-G50 microcolumn gel card for Brucella serum agglutination test

doi:10.3969/j.issn.1673-8640.2020.11.021

Abstract

Abstract:

Objective To investigate homemade microcolumn gel card for Brucella serum agglutination test. Methods For 160 suspected patients with Brucella infection,3 different specifications of Sephadex-G50 microcolumn gel particles were used as fillers to make homemade microcolumn gel cards for Brucella serum agglutination test. Serum tube agglutination test was used as the gold standard for application role comparison. Results The microcolumn gel card prepared with G50-Fine gel dry powder was compared with serum tube agglutination test. The authenticity,reliability and correlation of serum agglutination titer were satisfactory. Conclusions The microcolumn gel card prepared by G50-Fine gel dry powder is suitable for Brucella serum agglutination test. The method is easy to measure,accurate and reliable,and has a short detection time. It has good clinical application value.

Key words: Microcolumn gel detection technology, Brucella, Serum tube agglutination test

CLC Number:

R446.1

LI Yan, GENG Huijuan, QIU Siyuan, WANG Xianghua, WANG Hao. Homemade Sephadex-G50 microcolumn gel card for Brucella serum agglutination test[J]. Laboratory Medicine, 2020, 35(11): 1173-1176.

Figures/Tables 4

References 11

[1]	GALIŃSKA E M,ZAGÓRSKI J. Brucellosis in humans-etiology,diagnostics,clinical forms[J]. Ann Agic Environ Med,2013,20(2):233-238.
[2]	LEMNOUER A,FRIKH M,MALEB A,et al.Brucellosis:a cause of meningitis not to neglect[J]. IDCases,2017,10:97-99.
[3]	FRANC K A,KRECEK R C,HÄSLER B N,et al. Brucellosis remains a neglected disease in the developing world:a call for interdisciplinary action[J]. BMC Public Health,2018,18(1):125.
[4]	中华人民共和国国家卫生健康委员会. WS 269—2019 布鲁氏菌病诊断[S]. 2019.
[5]	JORGENSEN J H.临床微生物学手册[M]. 11版. 北京:中华医学电子音像出版社,2017.
[6]	尚红,王毓三,申子瑜. 全国临床检验操作规程[M]. 4版. 北京:人民卫生出版社,2015.
[7]	IRVEM A,YÜCEL F M,AKSARAY S,et al. Comparison of a new and rapid method,Brucella Coombs gel test with the other methods in the serological diagnosis of brucellosis[J]. Mikrobiyoloji Bul,2015,49(2):181-187.
[8]	PORATH J,FLODIN P.Gel filtration:a method for desalting and group separation[J]. Nature,1959,183(4676):1657-1659.
[9]	LAPIERRE Y,RIGAL D,ADAM J,et al.The gel test:a new way to detect red cell antigen-antibody reactions[J]. Transfusion,1990,30(2):109-113.
[10]	康勇,罗茜. 液体过滤与过滤介质[M]. 北京:化学工业出版社,2008.
[11]	王兰兰. 临床免疫学和免疫检验[M]. 北京:人民卫生出版社,2004.

凝胶干粉类型	球蛋白的分离范围（相对分子质量）	右旋糖酐的分离范围（相对分子质量）	近似的柱床体积/ （kg/mL）	凝胶颗粒近似直径/μm	20 ℃溶胀时间/h
G50-Superfine	1 500~30 000	500~10 000	9~11	20~50	3
G50-Fine	1 500~30 000	500~10 000	9~11	20~80	3
G50-Medium	1 500~30 000	500~10 000	9~11	50~150	3

凝胶干粉类型	球蛋白的分离范围（相对分子质量）	右旋糖酐的分离范围（相对分子质量）	近似的柱床体积/ （kg/mL）	凝胶颗粒近似直径/μm	20 ℃溶胀时间/h
G50-Superfine	1 500~30 000	500~10 000	9~11	20~50	3
G50-Fine	1 500~30 000	500~10 000	9~11	20~80	3
G50-Medium	1 500~30 000	500~10 000	9~11	50~150	3

凝胶卡编号（凝胶干粉型号）	微柱凝胶血清凝集试验结果	试管凝集试验结果/例		真实性评价					可靠性评价
凝胶卡编号（凝胶干粉型号）	微柱凝胶血清凝集试验结果	+	-	敏感性/ %	特异性/ %	假阴性率/ %	假阳性率/ %	约登指数	符合率/ %	Kappa值
A（G50-Superfine）	+	67	17	100	81.72	0	18.28	0.82	89.38	0.79
	-	0	76
B（G50-Fine）	+	64	5	95.52	94.62	4.48	5.38	0.90	95.00	0.90
	-	3	88
C（G50-Medium）	+	42	0	46.27	100	53.73	0	0.46	77.50	0.50
	-	25	93

凝胶卡编号（凝胶干粉型号）	微柱凝胶血清凝集试验结果	试管凝集试验结果/例		真实性评价					可靠性评价
凝胶卡编号（凝胶干粉型号）	微柱凝胶血清凝集试验结果	+	-	敏感性/ %	特异性/ %	假阴性率/ %	假阳性率/ %	约登指数	符合率/ %	Kappa值
A（G50-Superfine）	+	67	17	100	81.72	0	18.28	0.82	89.38	0.79
	-	0	76
B（G50-Fine）	+	64	5	95.52	94.62	4.48	5.38	0.90	95.00	0.90
	-	3	88
C（G50-Medium）	+	42	0	46.27	100	53.73	0	0.46	77.50	0.50
	-	25	93