Abstract:

Objective To evaluate the consistency of multiplex flow immunoassay and traditional methods in the determinations of infant ToRCH specific antibodies [toxoplasma（TOX）,rubella（RUB）,cytomegalovirus（CMV） and herpes simplex virus（HSV）]. Methods ToRCH-IgM and ToRCH-IgG antibodies were determined by multiplex flow immunoassay in 130 infants,and the results were compared with those of chemiluminescence. The samples with inconsistent results were further determined by enzyme-linked immunosorbent assay（ELISA）. Results Except for CMV IgG antibody,multiplex flow immunoassay and chemiluminescence had good consistency,and the consistency rate was >80.00%（K>0.4）. However,for CMV IgG antibody,the consistency rates of multiplex flow immunoassay with chemiluminescence and ELISA were 45.37% and 47.22%（K=0.10 and 0.11）. Conclusions There is a good consistency for TOX antibodies（IgM and IgG）,RUB antibodies（IgM and IgG）,CMV antibody（IgM）and HSV antibodies（IgM and IgG） between multiplex flow immunoassay and traditional methods（chemiluminescence and ELISA）,while there is difference for CMV antibody （IgG）between chemiluminescence and ELISA.

Key words: Multiplex flow immunoassay, ToRCH specific antibodies, Infants