Application on Feulgen-EA staining fixative

doi:10.3969/j.issn.1673-8640.2017.08.010

Abstract

Abstract:

Objective To investigate Feulgen-EA staining fixative primarily. Methods By image analysis,cells were scanned after fixation by 5 fixatives [glacial acetic acid（acetic acid）,AF,improved AF,Clarke and Bohm-Sprenger],nucleus integrated optical density（IOD）,coefficient of variation（CV）and area were compared,and suitable DNA fixative was obtained. The influence of temperature on fixation was evaluated. The effect of cytoplasmic staining was compared by EA staining. Results DNA fixation may choose appropriately AF or Bohm-Sprenger fixed-liquid reaction for 30 min. When temperature was too low,it may influence on DNA fixation,and the effect of EA to cytoplasmic staining was good. Conclusions AF and Bohm-Sprenger fixatives are suitable for Feulgen-EA double staining.

Key words: Staining and labeling, Pathology, Image processing, Computer assistance, Fixative, Feulgen-EA staining

CLC Number:

R446.1

HUANG Rongxiang, YU Lanlan, ZHU Chenyan, HE Wenbo, MA Liang, NIE Kun, ZHANG Jing. Application on Feulgen-EA staining fixative[J]. Laboratory Medicine, 2017, 32(8): 704-708.

Figures/Tables 10

References 15

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