Alkali treatment method for improving the specificity of serum 1,3-beta-D-glucan determination

doi:10.3969/j.issn.1673-8640.2017.01.012

Abstract

Abstract:

Objective To study alkali treatment method for improving the specificity of serum 1,3-beta-D-glucan determination. Methods A total of 73 patients suspected with invasive fungal disease were enrolled from April 2013 to December 2014. Serum 1,3-beta-D-glucan level was determined by alkali treatment method and heating dilution method,respectively. The results of fungal culture were as evaluation standard. The results were analyzed by SPSS 19.0 software. Results The coincidence rates of alkali treatment method and heating dilution method with fungal culture were 87.50% and 63.64%,respectively. There was statistical significance between alkali treatment method and heating dilution method（P<0.05）. The consistency of the 2 treatment methods was not good（Kappa=0.718）. Conclusions Alkali treatment method could make 1,3-beta-D-glucan existing with a low structure form and also eliminating the effect of high structure,which improves the specificity of serum 1,3-beta-D-glucan determination. So, alkali treatment method is better than heating dilution method.

Key words: Alkali treatment method, Heating dilution method, 1,3-Beta-D-glucan, Specificity, Fungi

CLC Number:

R446.61

XUE Zhixin, LIU Juanjuan, WANG Baoxue, LI Yanan, SU Yan, ZHOU Zeqi. Alkali treatment method for improving the specificity of serum 1,3-beta-D-glucan determination[J]. Laboratory Medicine, 2017, 32(1): 48-51.

Figures/Tables 3

References 17

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加热稀释法	碱处理法		合计
加热稀释法	+	-	合计
+	15	7	22
-	1	50	51
合计	16	57	73

加热稀释法	碱处理法		合计
加热稀释法	+	-	合计
+	15	7	22
-	1	50	51
合计	16	57	73

真菌培养法	加热稀释法		合计
真菌培养法	+	-	合计
+	14	2	16
-	8	49	57
合计	22	51	73

真菌培养法	加热稀释法		合计
真菌培养法	+	-	合计
+	14	2	16
-	8	49	57
合计	22	51	73

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