Percentages of peripheral blood CD4+CD25+CD127low T cells before and after the treatment of paternal lymphocyte induced immunization in patients with unexplained recurrent spontaneous abortion

doi:10.3969/j.issn.1673-8640.2017.01.004

Abstract

Abstract:

Objective To investigate the expression of peripheral blood CD4⁺CD25⁺CD127^1ow T cells in regulatory T cells （Treg） of patients with unexplained recurrent spontaneous abortion（URSA）,to analyze the percentages before and after the treatment of paternal lymphocyte induced immunization,and to evaluate its probability as a diagnosis indicator for abortion. Methods By flow cytometry,the percentages of peripheral blood CD4⁺CD25⁺CD127^1ow T cells were determined in 73 patients with URSA before the treatment,76 healthy women with early pregnancy and 69 healthy women without pregnancy. A total of 51 patients with URSA underwent the treatment of paternal lymphocyte induced immunization for 2-3 treatment courses. Their percentages were determined as well. Results Before the treatment,the percentage of CD4⁺CD25⁺CD127^1ow T cells in URSA group （2.73%±0.56%） was lower than those in healthy pregnancy and non-pregnancy groups（4.36%±1.07% and 2.93%±0.60%）（P<0.05）. After the treatment,the percentage（3.72%±1.15%）was higher than that before the treatment（P<0.05）. The pregnancy outcome of 42 patients after the treatment was good,while the treatment for the other 9 patients failed. Conclusions The percentage of peripheral blood CD4⁺CD25⁺CD127^1ow T cells in URSA patients is lower than those in healthy pregnant and non-pregnant women,which can be as a diagnosis indicator for URSA,and it can be improved by the treatment of paternal lymphocyte induced immunization.

Key words: CD4⁺CD25⁺CD127^1ow T cells, Unexplained recurrent spontaneous abortion, Paternal lymphocyte induced immunization treatment

CLC Number:

R446.1

LI Cui, SHI Youhao, WU Jiangnan, YING Chunmei. Percentages of peripheral blood CD4⁺CD25⁺CD127^low T cells before and after the treatment of paternal lymphocyte induced immunization in patients with unexplained recurrent spontaneous abortion[J]. Laboratory Medicine, 2017, 32(1): 14-17.

Figures/Tables 3

References 15

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组别	例数	CD4⁺CD25⁺CD127^lowT细胞	CD4⁺CD25⁺T细胞^△
URSA组	73	2.73±0.56	9.41±3.46
治疗前	51	2.74±0.57	9.66±3.57
治疗后	51	3.72±1.15^#	13.03±4.20^#
正常早孕组	76	4.36±1.07^*	13.41±4.08^*
正常未孕组	69	2.93±0.60^*	10.66±3.85^*

组别	例数	CD4⁺CD25⁺CD127^lowT细胞	CD4⁺CD25⁺T细胞^△
URSA组	73	2.73±0.56	9.41±3.46
治疗前	51	2.74±0.57	9.66±3.57
治疗后	51	3.72±1.15^#	13.03±4.20^#
正常早孕组	76	4.36±1.07^*	13.41±4.08^*
正常未孕组	69	2.93±0.60^*	10.66±3.85^*

项目	妊娠结局（例）			成功率（%）
项目	治疗成功	治疗失败	合计	成功率（%）
阳性	37	3	40	92.5
阴性	5	6	11	45.5
合计	42	9	51	82.4

项目	妊娠结局（例）			成功率（%）
项目	治疗成功	治疗失败	合计	成功率（%）
阳性	37	3	40	92.5
阴性	5	6	11	45.5
合计	42	9	51	82.4