Establishment on the reference intervals of serum free κ light chain, free λ light chain and their ratio in the population over 60 years old

doi:10.3969/j.issn.1673-8640.2016.12.005

Abstract

Abstract:

Objective To establish the reference intervals of serum free kappa（κ） light chain,free lambda（λ） light chain and their ratio in the population over 60 years old.Methods Epidemiological data（the histories of cardiovascular,hepatic,renal and diabetes mellitus diseases and pharmacohistory） were collected from 342 elder healthy subjects（227 cases of derivation cohort and 115 cases of validation cohort） in communities. Serum free κ light chain,free λ light chain,total κ light chain and total λ light chain were determined,and the ratio of free κ light chain to free λ light chain（free κ/λ ratio） was calculated. The results were classified and analyzed according to sex and age. The bilateral 2.5th and 97.5th percentiles were used to establish reference intervals,and the validation cohort's distribution of free κ/λ ratio in reference intervals was evaluated. Results There was statistical significance for hemoglobin,creatinine and calcium between males and females（P<0.05）. There was no statistical significance for total κ light chain,total λ light chain,the ratio of total κ light chain to total λ light chain（total κ/λ ratio）,free κ light chain,free λ light chain and free κ/λ ratio between males and females with different ages（P>0.05）. There was no correlation of free κ/λ ratio with hemoglobin,creatinine and calcium（r=0.046,-0.071 and 0.054,P>0.05）. According to the bilateral 2.5th and 97.5th percentiles,the reference intervals of free κ light chain,free λ light chain and free κ/λ ratio were 7.17-14.07 mg/L,23.04-60.59 mg/L and 0.15-0.46. There were 95.65%,95.65% and 96.52% subjects' free κ light chain,free λ light chain and free κ/λ ratio in validation cohort within the above 3 reference intervals. Conclusions The reference intervals of free κ light chain,free λ light chain and free κ/λ ratio in the population over 60 years old have been established,which can be used in the population over 60 years old.

Key words: Free kappa light chain, Free lambda light chain, Free kappa light chain to free lambda light chain ratio, Reference intervals

CLC Number:

R446.1

LI Ping, WU Jiong, PAN Baishen. Establishment on the reference intervals of serum free κ light chain, free λ light chain and their ratio in the population over 60 years old[J]. Laboratory Medicine, 2016, 31(12): 1031-1035.

性别	例数	年龄（岁）	血红蛋白（g/L）	肌酐（μmol/L）	钙（mmol/L）	游离κ轻链（mg/L）
男性	89	70.9±5.6	147.3±11.0	77.9±12.4	2.37±0.08	10.5±1.79
女性	138	68.5±5.4	133.2±15.6	59.6±11.8	2.39±0.08	10.3±1.50
P值			<0.001	<0.001	<0.05	>0.05
性别	游离λ轻链（mg/L）		游离κ/λ比值	总κ轻链（g/L）	总λ轻链（g/L）	总κ/λ比值
男性	34.09±9.54		0.33±0.09	3.46±0.64	1.94±0.34	1.80±0.28
女性	34.10±8.20		0.32±0.07	3.42±0.69	1.98±0.46	1.77±0.32
P值	>0.05		>0.05	>0.05	>0.05	>0.05

性别	例数	年龄（岁）	血红蛋白（g/L）	肌酐（μmol/L）	钙（mmol/L）	游离κ轻链（mg/L）
男性	89	70.9±5.6	147.3±11.0	77.9±12.4	2.37±0.08	10.5±1.79
女性	138	68.5±5.4	133.2±15.6	59.6±11.8	2.39±0.08	10.3±1.50
P值			<0.001	<0.001	<0.05	>0.05
性别	游离λ轻链（mg/L）		游离κ/λ比值	总κ轻链（g/L）	总λ轻链（g/L）	总κ/λ比值
男性	34.09±9.54		0.33±0.09	3.46±0.64	1.94±0.34	1.80±0.28
女性	34.10±8.20		0.32±0.07	3.42±0.69	1.98±0.46	1.77±0.32
P值	>0.05		>0.05	>0.05	>0.05	>0.05

年龄	例数	总κ轻链（g/L）	总λ轻链（g/L）	总κ/λ比值	游离κ轻链（mg/L）	游离λ轻链（mg/L）	游离κ/λ比值
60~70岁	130	3.5±0.5	2.0±0.4	1.8±0.3	10.2±1.5	33.7±8.7	0.3±0.08
>70岁	97	3.5±0.6	2.0±0.5	1.8±0.3	10.7±1.7	34.7±8.8	0.3±0.08
P值		>0.05	>0.05	>0.05	>0.05	>0.05	>0.05

年龄	例数	总κ轻链（g/L）	总λ轻链（g/L）	总κ/λ比值	游离κ轻链（mg/L）	游离λ轻链（mg/L）	游离κ/λ比值
60~70岁	130	3.5±0.5	2.0±0.4	1.8±0.3	10.2±1.5	33.7±8.7	0.3±0.08
>70岁	97	3.5±0.6	2.0±0.5	1.8±0.3	10.7±1.7	34.7±8.8	0.3±0.08
P值		>0.05	>0.05	>0.05	>0.05	>0.05	>0.05

项目	肌酐	血红蛋白	钙	总κ轻链	总λ轻链
游离κ轻链
r值	0.068	0.008	0.033	0.402
P值	>0.05	>0.05	>0.05	<0.01
游离λ轻链
r值	0.179	-0.049	-0.04		-0.211
P值	<0.01	>0.05	>0.05		<0.01
游离κ/λ比值
r值	-0.071	0.046	0.054
P值	>0.05	0.05	0.05

Establishment on the reference intervals of serum free κ light chain, free λ light chain and their ratio in the population over 60 years old

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1	陈文明. 多发性骨髓瘤的诊断与鉴别诊断[J]. 中国实用内科杂志,2006,26(12):889-891.
2	KOSMAS C,STAMATOPOULOS K,STAVROYIANNI N,et al. Molecular analysis of immunoglobulin genes in multiple myeloma[J]. Leuk Lymphoma,1999,33(3-4): 253-265.
3	BHOLE MV,SADLER R,RAMASAMY K. Serum-free light-chain assay: clinical utility and limitations[J]. Ann Clin Biochem,2014,51(Pt 5):528-542.
4	KATZMANN JA,CLARK RJ,ABRAHAM RS,et al. Serum reference intervals and diagnostic ranges for free kappa and free lambda immunoglobulin light chains:relative sensitivity for detection of monoclonal light chains[J]. Clin Chem,2002,48(9):1437-1444.
5	李华,翟锬. 血清κ/λ轻链比值测定对多发性骨髓瘤的诊断价值[J]. 国际检验医学杂志,2006,27(2):100-101.
6	隋伟薇,姚宏静,王亚非,等. 多发性骨髓瘤患者血清游离轻链检测及其临床意义[J]. 中华医学杂志,2007,87(44):3158-3160.

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