Abstract:

Objective To investigate the influence of hemoglobin H （HbH） disease on 4 kinds of glycated hemoglobin A_1c（HbA_1c） systems and the applicability of HbA_1c for patients with HbH disease. Methods A total of 27 samples with HbH disease and 30 control samples were collected. HbA_1c determinations were performed by boronate affinity high performance liquid chromatography,capillary electrophoresis,turbidimetric immunoassay and ion exchange high performance liquid chromatography. The difference of the 4 systems was compared. Fasting glucose（FG） and glycated serum protein（GSP） were determined as well. The differences of FG,GSP,HbA_1c and average glucose were compared between control and HbH disease groups. Results Compared with boronate affinity high performance liquid chromatography（Ultra²）,P values were 0.08,0.89 and 0.64,with no statistical significance（P>0.05）. The differences of FG and GSP had no statistical significance between control and HbH disease groups（P>0.05）. The differences of HbA_1c and average glucose had statistical significance between control and HbH disease groups（P<0.01）. Conclusions HbA_1c of HbH disease group can not reflect the real level of average glucose,and it is not applicable for long-term blood glucose monitoring in patients with HbH disease.

Key words: Glycated hemoglobin A1c, Hemoglobin H, Red blood cell life span, Average glucose