Table of Content

30 January 2024, Volume 39 Issue 1

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Correlation between serum Trx2，P-tau and total magnetic resonance imaging load score and cognitive function in patients with cerebral small vessel disease

DUAN Pengcang, DONG Ruifang, LIU Xiaoman, CAO Yang, WANG Ruoxi

2024, 39(1):  1-6.  DOI: 10.3969/j.issn.1673-8640.2024.01.001

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Objective To investigate the correlation between serum thioredoxin 2（Trx2），phosphorylated tau（P-tau） and total magnetic resonance imaging（MRI） load score and cognitive function in patients with cerebral small vessel disease. Methods Totally，94 patients with cerebral small vessel disease in Cangzhou Central Hospital from July 2019 to July 2020 were enrolled. According to whether they had cognitive function impairment，the patients were classified into cognitive impairment group（38 cases） and non-cognitive impairment group（56 cases）. According to the total MRI load score，the patients were classified into mild load group（67 cases） and moderate to severe load group（27 cases）. The general data and clinical laboratory determination results were collected. Serum Trx2 and P-tau were determined，and the factors for total MRI load and cognitive function in patients with cerebral small vessel disease was analyzed by multivariant Logistic regression analysis. Pearson correlation analysis was used to evaluate the correlation between each indicator and total MRI load and cognitive function. Results There was statistical significance in sex，age，drinking history，smoking history，high-density lipoprotein cholesterol（HDL-C），uric acid（UA），total MRI load score，Trx2 and P-tau levels between cognitive impairment group and non-cognitive impairment group（P<0.05），and there was no statistical significance in the other indicators（P>0.05）. There was statistical significance in sex，age，hypertension history，HDL-C，UA，Trx2 and P-tau levels between mild load group and moderate to severe load group（P<0.05），and there was no statistical significance in the other indicators（P>0.05）. Serum Trx2 [odds ratio（OR）=2.751，95% confidence interval（CI） 1.123-6.738] and serum P-tau（OR=2.651，95%CI 1.180-5.956） were independent risk factors for cognitive function impairment in patients with cerebral small vessel disease（P<0.05）. Serum Trx2 and P-tau were positively correlated with total MRI load score and CMMSE score in patients with cerebral small vessel disease（P<0.05）. Conclusions Serum Trx2 and P-tau are related to total MRI load and cognitive function in patients with cerebral small vessel disease，which can be used to evaluate the severity and cognitive function of cerebral small vessel disease.

Roles of serum IL-26 and IL-27 levels in patients with colorectal cancer

LAN Jun, LU Shuting, YANG Jianrui

2024, 39(1):  7-12.  DOI: 10.3969/j.issn.1673-8640.2024.01.002

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Objective To investigate the roles of serum interleukin（IL）-26 and IL-27 levels in patients with colorectal cancer. Methods A total of 63 patients with colorectal cancer（colorectal cancer group），63 patients with rectal polyps（rectal polyp group） and 63 healthy subjects（healthy control group） were enrolled from Hanzhong No. 3201 Hospital from May 2018 to May 2021. The levels of IL-26，IL-27，carcinoembryonic antigen（CEA） and carbohydrate antigen 19-9（CA19-9） were determined. The general data and clinical pathological data were collected. The efficacy of each indicator for the diagnosis of colorectal cancer was evaluated by receiver operating characteristic（ROC） curve. All the patients with colorectal cancer were followed up for 3 years，and the survival status was assessed by Kaplan-Meier survival curve. Results Serum levels of IL-26 and IL-27 were progressively higher in colorectal cancer group，rectal polyp group and healthy control group（P<0.001）. There was statistical significance in serum IL-26 and IL-27 levels among patients with colorectal cancer with or without lymphatic metastasis，different TNM stages and different degrees of differentiation（P<0.05）. There was no statistical significance for serum IL-26 and IL-27 levels in different ages，sex，presence or absence of distant metastasis，pathological types and tumor sizes of colorectal cancer（P>0.05）. The areas under curves（AUC） of single and combined determinations of IL-26，IL-27，CEA and CA19-9 in diagnosing colorectal cancer were 0.82，0.86，0.81，0.82 and 0.92，respectively. Based on the optimal cut-off values obtained from the ROC curves，the patients with colorectal cancer were classified into high IL-26 level group（41 cases），low IL-26 level group（22 cases） and high IL-27 level group（43 cases） ，low IL-27 level group（20 cases），respectively. The progression-free survival periods of high IL-26 and high IL-27 level groups were shorter than those of low IL-26 and low IL-27 level groups（P<0.01）. Conclusions Serum levels of IL-26 and IL-27 are abnormally elevated in patients with colorectal cancer and can be used in combination with tumor markers for the auxiliary diagnosis and prognosis assessment of colorectal cancer.

Expression and prognostic assessment value of miR-924 and SLC1A5 in lung cancer tissues

ZHANG Miaomiao, DUAN Dongkui, YU Chun, WANG Lina

2024, 39(1):  13-18.  DOI: 10.3969/j.issn.1673-8640.2024.01.003

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Objective To investigate the prognostic assessment roles of miR-924 and solute carrier family 1 member 5（SLC1A5） in lung cancer tissues. Methods From February 2018 to February 2019，97 lung cancer patients from Nanyang Central Hospital were enrolled，and the general data and cancer and adjacent tissues（>2 cm from tumor edges） were collected. The miR-924 and SLC1A5 mRNA and SLC1A5 expressions were determined. The correlation between miR-924 and SLC1A5 mRNA was analyzed by Pearson correlation analysis. Kaplan-Meier survival curve was used，and Cox regression analysis was used to analyze the risk factors for the poor prognosis of lung cancer patients. Results Compared with adjacent tissue group，the relative expression level of miR-924 in cancer tissue group was decreased（P<0.001），while that of SLC1A5 mRNA and the positive rate of SLC1A5 were increased（P<0.001）. The miR-924 expression was negatively correlated with SLC1A5 mRNA in lung cancer tissue（r=-0.843，P<0.05）. The research subjects were classified into high-expressed group，low-expressed group and positive group，negative group according to the mean value of miR-924 relative expression level or the expression of SLC1A5，respectively. There was statistical significance in the differentiation degree and clinical stage between miR-924 low-expressed group and high-expressed group and between SLC1A5 positive group and negative group（P<0.05）. The cumulative survival rate of miR-924 high-expressed group was higher than that of miR-924 low-expressed group（Log-rank χ²=5.453，P<0.05），and the cumulative survival rate of SLC1A5 positive group was lower than that of SLC1A5 negative group（Log-rank χ²=9.259，P<0.05）. Clinical stage Ⅲ，low expression of miR-924 and positive SLC1A5 were risk factors for poor prognosis of lung cancer patients（P<0.05）. Conclusions The lung cancer patients has abnormal expressions of miR-924 and SLC1A5 in cancer tissue，which are expected to become the markers for evaluating lung cancer prognosis.

Predictive value of soluble CD163 combined with Charlson index for esophageal variceal bleeding in patients with liver cirrhosis

ZHANG Yang, ZHANG Dehe, TANG Shiyue, ZHANG Jun, WANG Jianming, CHEN Ling

2024, 39(1):  19-25.  DOI: 10.3969/j.issn.1673-8640.2024.01.004

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Objective To investigate the predictive value of soluble CD163（sCD163） combined with Charlson index for esophageal variceal bleeding in patients with liver cirrhosis. Methods Totally，100 patients with liver cirrhosis from Jinhua Hospital of Zhejiang University School of Medicine from April 2016 to April 2018 were enrolled as modeling group. The general data and clinical laboratory determination results were collected. According to whether esophageal variceal bleeding occurred during the follow-up period，they were classified into bleeding group（32 cases） and non-bleeding group（68 cases）. Another 50 patients with liver cirrhosis from Jinhua Hospital of Zhejiang University School of Medicine from June 2018 to June 2020 were enrolled as validation group. Logistic regression analysis was used to evaluate the risk factors for esophageal variceal bleeding in patients with liver cirrhosis. A column chart model was established，and a validation group for external validation was used. Receiver operating characteristic（ROC） curve and calibration curve were used to evaluate the discrimination and accuracy of the column chart model. ROC curve was used to evaluate the efficacy of single and combined determinations of sCD163 and Charlson index in determining esophageal variceal bleeding in patients with liver cirrhosis. Results There was no statistical significance in general data and clinical laboratory determination results between modeling group and validation group（P>0.05）. The incidence of hepatic ascites，degree of esophageal varices，red sign，portal vein diameter，splenic vein diameter，Charlson index，prothrombin time（PT），sCD163 and D-dimer（DD） levels in bleeding group were higher than those in non-bleeding group（P<0.05），and there was no statistical significance in the other indicators between the 2 groups（P>0.05）. The presence of hepatic ascites，severe esophageal varices，red sign and increased portal vein diameter，sCD163 and Charlson index were risk factors for esophageal variceal bleeding in patients with liver cirrhosis [odds ratios（OR） were 2.124 ， 1.865 ， 2.001 ， 2.412 ，1.685 and 2.623 ，95% confidence intervals（CI） were 1.235-3.024 ， 1.425-2.563 ， 1.121-3.221 ， 1.785-3.500 ， 1.247-2.875 and 2.013-3.245，respectively]. The areas under curves for single and combined determinations of sCD163 and Charlson index to determine esophageal variceal bleeding in patients with liver cirrhosis were 0.846，0.852 and 0.889，respectively. The ROC curve and calibration curve both showed that the constructed column chart model had good discrimination and accuracy. Conclusions The combined determination of sCD163 and Charlson index has good predictive value for esophageal variceal bleeding in patients with liver cirrhosis.

Serum amphiregulin and mesothelin in auxiliary diagnosis of breast cancer

LIU Bohan, LIU Yiwen, HE Yiqing, LU Renquan, DU Yan, ZHANG Guoliang, GUO Qian, GAO Feng, YANG Cuixia

2024, 39(1):  26-30.  DOI: 10.3969/j.issn.1673-8640.2024.01.005

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Objective To investigate the roles of serum amphiregulin（AREG） and mesothelin（MSLN） levels in the auxiliary diagnosis of breast cancer. Methods A total of 54 female patients with breast cancer（breast cancer group），38 patients with benign breast lesions（benign lesion group） and 35 apparently healthy subjects（healthy control group） were enrolled from the Cancer Hospital of Fudan University from December 2018 to May 2019. Serum AREG and MSLN levels were determined by enzyme-linked immunosorbent assay. The relationship between serum AREG and MSLN levels and clinicopathological characteristics of breast cancer patients was analyzed. The efficacy of AREG and MSLN alone and in combination with carcinoembryonic antigen（CEA） and carbohydrate antigen（CA）15-3 in the auxiliary diagnosis of breast cancer was evaluated by receiver operating characteristic（ROC） curve. Results Compared with healthy control group，serum AREG and MSLN levels in breast cancer group were increased（P<0.05）. The areas under curves（AUC） of AREG and MSLN in the diagnosis of breast cancer were 0.582 and 0.637，respectively，while the AUC of serum AREG and MSLN combined with CEA and CA15-3 was 0.757. Serum AREG and MSLN levels in breast cancer group were related with pathological types and lymphatic metastasis（P<0.05）. Conclusions Serum AREG，MSLN combined with CEA and CA15-3 can play a role in the auxiliary diagnosis of breast cancer.

Correlation between lncRNA MIR155HG and pulmonary function in patients with COPD and its auxiliary diagnosis value for AECODP

ZHANG Feifei, WANG Jingwen, ZHANG Yuexin, ZHENG Dawei, MEN Xiang

2024, 39(1):  31-36.  DOI: 10.3969/j.issn.1673-8640.2024.01.006

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Objective To investigate the correlation between long non-coding RNA（lncRNA） MIR155HG and pulmonary function in patients with chronic obstructive pulmonary disease（COPD） and its auxiliary diagnosis value for acute exacerbation of chronic obstructive pulmonary disease（AECOPD）. Methods A total of 117 COPD patients treated in Nanyang Central Hospital from October 2018 to March 2021 were enrolled，which included 60 patients in stable phase as stable phase group and 57 patients with AECOPD as AECOPD group，and 60 healthy subjects were enrolled as control group. The general data were collected. Pulmonary function [forced expiratory volume in one second（FEV₁），forced vital capacity（FVC）] was evaluated，and FEV₁/FVC% was calculated. The level of lncRNA MIR155HG in peripheral blood mononuclear cell（PBMC） was determined. Logistic regression analysis was used to analyze the factors of AECOPD. Receiver operating characteristic（ROC） curve was used to analyze the efficiency of various indicators in the diagnosis of AECOPD. Results White blood cell（WBC）count，neutrophil percentage（NEUT%），procalcitonin（PCT），C-reactive protein（CRP），fibrinogen（Fib） and lncRNA MIR155HG level in PBMC were decreased in turn in AECOPD group，stable phase group and control group（P<0.05）. FEV₁ and FEV₁/FVC% were increased in turn（P<0.05）. The level of lncRNA MIR155HG in PBMC in AECOPD group and stable phase group was positively correlated with WBC count，NEUT%，PCT，CRP and Fib（P<0.001），and they were negatively correlated with FEV₁ and FEV₁/FVC%（P<0.001）. Increased lncRNA MIR155HG and decreased FEV₁ were risk factors for AECOPD [odds ratios（OR）were 2.381，0.682，95% confidence intervals（CI）were 1.526-3.715，0.531-0.876]. The areas under curves（AUC） of FEV₁ and lncRNA MIR155HG single and combined determinations to predict AECOPD were 0.826，0.854 and 0.939，respectively. Conclusions The increased level of lncRNA MIR155HG in PBMC of COPD patients is related to clinical indicators and pulmonary function，which is expected to become an early predictive marker of AECOPD.

Development and evaluation of internal quality control materials for immunofixation electrophoresis

AN Chongwen, CHEN Jian, PANG Lu, XU Dong

2024, 39(1):  37-42.  DOI: 10.3969/j.issn.1673-8640.2024.01.007

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Objective To prepare internal quality control materials for agarose gel immunofixation electrophoresis（IFE），and to evaluate its homogeneity，stability and applicability. Methods Clinical samples were selected according to the related guidance for internal quality control materials. The negative，weak positive and positive quality control materials were made. The homogeneity，stability and applicability were evaluated. Results The homogeneity of quality control materials at 3 levels was good. The quality control materials were stored at 2-8 ℃ after opening for 4 months，and the stability was good. The quality control materials at -40 ℃ without opening had good consistency within 5 years. It had been used in other determination systems of the same brand and different determination systems of different brands，and the results were consistent. Conclusions The internal quality control materials for agarose gel IFE has good homogeneity，stability and applicability，which can meet the quality control requirements of clinical laboratories.

Roles of TAT，PIC and thromboelastography for assessing coagulation function in tumor patients

LAI Yuanyuan, HE Zhenye, SHEN Huaqing, LIN Yiteng, LIU Xijun, QI Jun, LIN Yongping

2024, 39(1):  43-46.  DOI: 10.3969/j.issn.1673-8640.2024.01.008

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Objective To analyze the roles of thrombin-antithrombin complex（TAT），plasmin-alpha 2-antiplasmin inhibitor complex（PIC） and thromboelastography（TEG） for assessing coagulation function in tumor patients. Methods A total of 120 tumor patients in Cancer Hospital and Shenzhen Hospital of Chinese Academy of Medical Sciences and Peking Union Medical College from January 2022 to August 2023 were enrolled as case group，and 30 healthy subjects were enrolled as control group. The difference of TAT，PIC and TEG were compared. Caprini score was used to compare the differences of TAT，PIC and TEG for the patients with different thrombus risk，and the differences of TAT，PIC and TEG with different tumor types were compared. The efficacy of TAT，PIC and TEG in coagulation function of tumor patients was analyzed by receiver operating characteristic（ROC） curve. Results In case group，TAT and PIC were higher than those in control group（P<0.05）. In thrombus extremely high-risk group，TAT and PIC were higher than those in medium/low-risk group（P<0.05）. TAT and PIC in patients with liver cancer，lung cancer，colon cancer and gastric cancer were increased（P>0.05）. The sensitivities of TAT，PIC and TEG in evaluating the hypercoagulable state of tumor patients were 85.0%，55.0% and 56.70%，and the specificities were 93.3%，81.7% and 70.0%，respectively. The areas under curves were 0.94，0.72 and 0.63，respectively. Conclusions TAT and PIC can determine abnormal coagulation function in tumor patients earlier than TEG，which can better indicate the hypercoagulabe state of tumor patients，and can be used as laboratory parameters for assessing coagulation function in tumor patients.

Drug resistance of Bacteroides isolated from clinic and characteristics of Bacteroides fragilis bft genotyping

WANG Yanyan, WANG Junrui, ZHENG Wenqi, LAN Haixia, GUO Sufang

2024, 39(1):  47-52.  DOI: 10.3969/j.issn.1673-8640.2024.01.009

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Objective To investigate the distribution and drug resistance characteristics of Bacteroides，and to investigate the genotyping characteristics of bft gene in Bacteroides fragilis. Methods Totally，147 isolates of Bacteroides were collected from the Affiliated Hospital of Inner Mongolia Medical University from June 2016 to June 2019，and they were identified by in vitro drug susceptibility test. Polymerase chain reaction（PCR） was used to determine bft gene in Bacteroides fragilis. Results In the 147 isolates of Bacteroides，100 isolates of Bacteroides fragilis were determined，accounting for 68.0%，25 isolates of Bacteroides thetaiotaomicron accounted for 17.0%，and 9 isolates of Bacteroides ovalis accounted for 6.1%. The highest drug resistance rate of Bacteroides was 78.9% to clindamycin. The clindamycin resistance rate for Bacteroides fragilis was 85.0%，which was higher than those of other Bacteroides（66.0%）. Imipenem and meropenem resistance rates were 13.6% and 4.7% for Bacteroides，respectively. Bacteroides showed different degrees of resistance to other drugs，and the most drug resistance rates of Bacteroides fragilis to drugs were higher than those of other Bacteroides. The positive rate of bft gene was 38.0% in Bacteroides fragilis. The main genotype was bft-1（28.0%），followed by bft-2（10.0%），and no bft-3 was found. The positive rate of bft gene of Bacteroides fragilis isolated from non-abdominal specimens（55.6%） was higher than that of Bacteroides fragilis isolated from abdominal specimens（36.3%）. Conclusions Bacteroides fragilis has the highest isolation rate in Bacteroides，and it has certain resistance to common clinical drugs. The prevalence of enterotoxin-producing isolates of Bacteroides fragilis isolated from non-abdominal specimens is higher，which should be widely paid attention in clinic.

Role of Group B Streptococcus detection in pregnant women combined with fetal cord blood neutrophil CD64 index for predicting neonatal infection with premature rupture of membranes

TAN Haiyan, JIANG Yurong

2024, 39(1):  53-59.  DOI: 10.3969/j.issn.1673-8640.2024.01.010

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Objective To investigate the role of Group B Streptococcus（GBS） detection in pregnant women combined with fetal cord blood neutrophil CD64 index for predicting neonatal infection with premature rupture of membranes（PROM）. Methods A total of 170 full-term PROM neonates in Hunan Maternal and Child Health Care Hospital from March 2022 to December 2022 were enrolled as observation group，and 170 full-term PROM neonates were classified into infected group（48 cases） and uninfected group（122 cases） according to neonatal infection. The general data of neonates were compared to analyze the relationship between neonatal PROM infection and clinical characteristics of neonates. The factors for neonatal PROM infection were analyzed by multivariant Logistic regression analysis. A line graph prediction model was constructed and verified. The predictive efficacy of GBS detection combined with fetal cord blood neutrophil CD64 index was evaluated by receiver operating characteristic（ROC） curve. Results The time from PROM to delivery，chorioamnionitis，GBS positivity，the number of vaginal finger examination，prenatal antibiotic usage，5 min Apgar score<7，amniotic fluid contamination and fetal cord blood neutrophil CD64 index had statistical significance between the 2 groups（P<0.05）. The time from PROM to delivery，chorioamnionitis，GBS positivity，the number of vaginal finger examination and fetal cord blood neutrophil CD64 index were the risk factors for neonatal PROM infection（P<0.05）. The total score of the above 5 indexes in the line graph prediction model was 294，corresponding to 73.15% of the risk of infection. The results of interaction analysis showed that fetal cord blood neutrophil CD64 index and GBS positivity had positive additive interaction on neonatal PROM infection（P<0.001）. The areas under curves of fetal cord blood neutrophil CD64 index and GBS positivity single and combined determinations were 0.763，0.807 and 0.847，respectively. Conclusions The detection of GBS in pregnant women combined with fetal cord blood neutrophil CD64 index has a high predictive value for neonatal PROM infection. The time from PROM to delivery，chorioamnionitis and the number of vaginal finger examination are risk factors for neonatal PROM infection，which should be paid attention in clinic to reduce the risk of infection.

Synergistic diagnosis of THR mRNA and ALCAT1 in hyperthyroidism heart disease

ZHANG Xiaoling, JIN Yao, LI Sufang, PENG Haiying

2024, 39(1):  60-67.  DOI: 10.3969/j.issn.1673-8640.2024.01.011

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Objective To investigate the roles of thyroid hormone receptor（THR） mRNA and acyl-coa：lysocardiolipin acyltransferase 1（ALCAT1） in the diagnosis of hyperthyroidism heart disease. Methods A total of 69 patients with hyperthyroidism heart disease in Zhangjiakou Maternal and Child Health Hospital from February 2020 to March 2022 were enrolled as hyperthyroidism heart disease group. Totally，69 patients with hyperthyroidism were enrolled as hyperthyroidism group，69 patients with non-hyperthyroidism heart disease were enrolled as non-hyperthyroidism heart disease group，and 69 healthy subjects were enrolled as healthy control group. The general data and determination results from all the patients were collected. THRα1 mRNA，THRβ1 mRNA and ALCAT1 were determined. Logistic regression analysis was used to analyze the relationship between THR mRNA，ALCAT1 levels and hyperthyroidism heart disease. Relative excess risk due to interaction（RERI），attribution percentage（AP） and synergy index（SI） were used to analyze the interaction between THR mRNA and ALCAT1 in the occurrence of hyperthyroidism heart disease. The efficacy of THR mRNA and ALCAT1 in the diagnosis of hyperthyroidism heart disease was evaluated by receiver operating characteristic（ROC） curve. Results The relative expression level of THRβ1 mRNA and ALCAT1 level in hyperthyroidism heart disease group were higher than those in hyperthyroidism group（P<0.001），and the relative expression level of THRα1 mRNA，THRβ1 mRNA and ALCAT1 level were higher than those in non-hyperthyroidism heart disease group and healthy control group（P<0.001）. The relative expression levels of THRα1 mRNA，THRβ1 mRNA and ALCAT1 level in hyperthyroidism heart disease group were higher than those in non-hyperthyroidism heart disease group and healthy control group（P<0.05），and the relative expression levels of THRα1 mRNA，THRβ1 mRNA and ALCAT1 level in non-hyperthyroidism heart disease group were higher than those in healthy control group（P<0.05）. After adjusting for hyperthyroidism course，free triiodothyronine（FT₃），free thyroxine（FT₄），thyroid-stimulating hormone（TSH），total triiodothyronine（TT₃） and total thyroxine（TT₄），elevated relative expression levels of THRβ1 mRNA and ALCAT1 level were risk factors for hyperthyroidism heart disease [odds ratios（OR） were 3.185 and 3.712，95% confidence intervals（CI） were 1.524-6.658 and 1.967-7.006，P<0.001]. There was a positive interaction between THRβ1 mRNA and ALCAT1 on the occurrence of hyperthyroidism heart disease，and the risk of hyperthyroidism heart disease was 9.000 times when both of them were highly expressed than when both of them were lowly expressed. The risk of hyperthyroidism heart disease caused by high expression of THRβ1 mRNA and ALCAT1 was 3.148 times than that of other unknown factors（OR=1）（RERI=3.148）. The synergistic effect was 1.855 times of the sum of the two effects alone（SI=1.855）. The 34.98%（AP=34.98%） of the risk of hyperthyroidism heart disease was due to their interaction. The area under curve（AUC） of single and combined determinations of THRβ1 mRNA and ALCAT1 in the diagnosis of hyperthyroidism heart disease were 0.798，0.726 and 0.872，respectively. Conclusions THRβ1 mRNA and ALCAT1 are independent risk factors of hyperthyroidism heart disease，and they have synergistic effect on the occurrence of hyperthyroidism heart disease. The combined determination can improve the clinical diagnostic efficiency of hyperthyroidism heart disease.

Promoting effect of miR-155-5p targeting ARID2 on oral squamous cell carcinoma

DAI Hongjian, CUI Haining, LI Yanfeng, CUI Hongmei

2024, 39(1):  87-94.  DOI: 10.3969/j.issn.1673-8640.2024.01.016

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Objective To investigate the regulation and mechanism of miR-155-5p on the proliferation，migration and invasion of oral squamous cell carcinoma（OSCC） cells. Methods Human OSCC cell lines CAL-27，HN4，HN6，SCC4，TCA8113 and normal oral epithelial keratinocyte line HOK were collected to determine the expression of miR-155-5p. According to the different transfection plasmids，they were classified into miR-NC group（transfected negative control with miR-155-5p mimics），miR-155-5p group（transfected with miR-155-5p mimics） and anti-NC group（transfected negative control with miR-155-5p inhibitor），anti-miR-155-5p group（transfected with miR-155-5p inhibitor），Vector group（transfected with empty plasmid vector），pc-ARID2 group（transfected with ARID2 overexpression plasmid），miR-NC+Vector group（co-transfected with negative control and empty plasmid vector），miR-155-5p+Vector group（co-transfected with miR-155-5p mimics and empty plasmid vector） and miR-155-5p+pc-ARID2 group（co-transfected with miR-155-5p mimics and ARID2 overexpression plasmid）. The cell proliferation was determined by CCK-8 assay and clonal formation assay，the migration and invasion of CAL-27 cells and HN4 cells were determined by scratch assay and invasion assay，and the expression of ARID2 protein was determined by western blot. Dual luciferase reporter gene assay was used to verify the targeting relationship between miR-155-5p and ARID2. Results Compared with oral epithelial keratinocyte line HOK，OSCC cell lines（SCC4，HN6，HN4，CAL-27，TCA8113） showed significantly up-regulated miR-155-5p expression（P<0.01）. Compared with anti-NC group，the relative expression of anti-miR-155-5p inhibitor group was significantly decreased（P<0.01）. The proliferation activity，clonal formation number，scratch closure rate and invasion cell number were decreased（P<0.01）. Compared with miR-NC group，the expression of ARID2 in miR-155-5p group was down-regulated（P<0.01），and the proliferation activity，clonal formation number，scratch closure rate and invasion cell number were increased（P<0.01）. The expression of ARID2 in anti-miR-155-5p inhibitor group was up-regulated（P<0.01），and the proliferation activity，clonal formation number，scratch closure rate and invasion cell number were decreased（P<0.01）. Compared with co-transfected miR-NC and pmiR-ARID2 wild-type plasmid（pmiR-ARID2-wt），the luciferase activity was decreased after co-transfecting miR-155-5p mimic and pmiR-ARID2 mutant-type plasmid（pmiR-ARID2-wt）（P<0.01）. Compared with Vector group，the expression of ARID2 mRNA in pc-ARID2 group was up-regulated（P<0.01），and the proliferation activity，clonal formation number，scratch closure rate and invasion cell number were decreased（P<0.01）. Compared with miR-NC+Vector group，the proliferation activity，clonal formation number，scratch closure rate and invasion cell number in miR-155-5p+Vector group were increased（P<0.01）. Compared with miR-155-5p+Vector group，the proliferation activity，clonal formation number，scratch closure rate and invasion cell number in miR-155-5p+pc-ARID2 group were decreased（P<0.01）. Conclusions The expression of miR-155-5p is up-regulated in OSCC cells，and it can promote the proliferation，migration and invasion of OSCC cells through targeted inhibition of ARID2 expression，thus playing the role of oncogene.

Homogenization management and practice of clinical blood and body fluid determinations

JIANG Haoqin, XU Qianqian, XU Liming, GUAN Ming

2024, 39(1):  95-99.  DOI: 10.3969/j.issn.1673-8640.2024.01.017

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The homogenization management of medical determination results is a basis for achieving mutual recognition of determination results and an important initiative to promote the sharing mechanism of medical services and enhance synergy among medical institutions. Due to the complexity and timeliness of disease changes，the differences in the performance of determination equipment and quality management of determination processes，as well as the difficulties of morphological determination，a series of medical risks may be brought about during the implementation of homogenization management. By analyzing the main problems that may exist in the pre-，mid- and post-analysis phases and the impact on determination results，preventive measures are analyzed to reduce or eliminate the risk factors affecting the homogenization of blood and body fluid. Combined with the professional characteristic technology，the clinical classic case practice of homogenization management in different medical institutions and multi-hospital areas is elaborated and analyzed.