与α-烯醇化酶相关的抗内皮细胞抗体ELISA的建立及临床初步应用
Establishment of a ELISA method for detecting anti-endothelial cell antibody related with human alpha-enolase and its primary clinical application
与α-烯醇化酶相关的抗内皮细胞抗体ELISA的建立及临床初步应用 |
| 肖艳群, 张健, 蒋玲丽, 陈慧英, 邵维杰, 简敏华, 陆银华 |
Establishment of a ELISA method for detecting anti-endothelial cell antibody related with human alpha-enolase and its primary clinical application |
| 图2 pET28a-ENOI纯化分析 注:1为纯化前;2为穿化介质后;3为第1次洗涤50 mmolL咪唑;4为第2次洗涤60 mmolL咪唑;5为第3次洗涤70 mmolL咪唑;6为洗脱;MW为Molecular weright marker |
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